PREPAKATION 01' MEDIA. , tS 



VIII. "Oiltay" Agar. 



1. Make a paste of 20 gms. of powdered agar in water 

 and add to 1,000 cc. of "Giltay" solution. 



2. Boil until dissolved. 



3. Filter through absorbent cotton. 



4. Sterilize in the autoclave as usual. 



IX. "Solution for Nitrite Formation." 



1. Dissolve the following materials in 1,000 cc. of distilled 

 water. 



1 gm. (NH,)2 SO.. 



1 gm. KH.PO.. 



0.5 gm. MgSOi. / 



0.4 gm. FeJSOs. 



2 gms. NaCl. ^ 



3. Stir until the solution is complete. 



3. Measure off 100 cc. portions in flasks. 



4. Add 1 gm. CaCOg to each portion. 



5. Sterilize in the autoclave at 15 lbs. pressure for ten 

 minutes. 



X. "Nitrite Solution for Nitrification." 



1. Dissolve in 1 liter (1,000 cc.) of distilled water: 



1 gm. NaNOj. 

 1 gm. NajCOa. 

 0.5 gm. KH,P04. 

 0.5 gm. NaOl. 

 0.4 gm. Fe^CSOJ^. 

 0.3 gm. MgSOj. 



2. Stir until all the materials are in solution. 



3. Sterilize in the autoclave as usual. 



