74 LABOKATOEY BACTERIOLOGY. 



XI. "Nitrate-Nitrogen Transformation" Solution. 



1. Dissolve in 1 liter (1,000 ce.) of distilled water : 



0.2 gm. K.HPO,. 

 0.2 gm. MgSO.. 

 0.5 gm. NaNOg. 

 0.02 gm. CaCl,. 

 1.0 gm. dextrose. 

 1 drop. sol. FezClc. 



2. Stir until dissolved. 



3. Add a few drops of phenolphthalein and then 10% 

 JSTaOH until the solution is Just neutral. 



4. Sterilize in the autoclave at 15 lbs. pressure for ten 

 minutes. 



XII. " Ammonior-Nitrogen Transformation" Solution. 



1. Dissolve in 1 liter of distilled water : 



0.2 gm. K^HPO.. 

 0.2 gm. MgSO,. 

 1.0 gm. (NH,)^ SO.. 

 0.02 gm. CaClj. 

 1.0 gm. dextrose. 

 1 drop sol. Fe^Clo. 



2. Stir until dissolved. 



3. Add 10% ISTaOH until the solution is neutral to phe- 

 nolphthalein. 



4. Sterilize in the autoclave as usual. 



