PEEPABATION OF MEDIA. 73 



XIII. "'Synthetic" Solution. 



1. To 1,000 ec. of tap water add the following : 



10 gms. dextrose. 

 0.2 gm. MgSOj. 

 0.5 gm. KjHPO,. 

 0.05 gm. peptone. 



3. Stir until dissolved. 



3. Sterilize in the autoclave as usual. 



XIV. "Synthetic" Agar. 



1. Make a paste of 20 gms. of powdered agar with cold 

 water and add to 1,000 cc. of "Synthetic Solution." 

 3. Boil until dissolved. 



3. Filter through absorbent cotton. 



4. Sterilize as usual. 



XV. "Cellulose" Solution. 



1. Dissolve in 1,000 cc. of tap water: 



0.5 (NHJ^PO,. 

 0.5 gm. KH^POj. 

 0.1 gm. MgSOi. 

 0.1 gm. NaCl. 



2. Sterilize in the autoclave as usual. 



