76 LABOILVTORY BACTERIOLOGY. 



XVI. "Badicicvia" Solution. 



1. Dissolve in 1,000 cc. of tap water : 



10 gms. saccharose. 

 1 gm. KjHPO,. 



2. Stir until dissolved. 



3. Sterilize in the autoclave at 10 lbs. pressure for 20 min- 

 utes. 



XVII. "Eadicicola" Agar. 



1. Make a paste of 20 gms. of powdered agar in cold 

 water and add to 1,000 cc. of "Eadicicola" solution. 



2. Boil until dissolved. 



3. Filter through absorbent cotton. 



4. Sterilize in the autoclave for twenty minutes at 10 lbs. 

 pressure. 



XVIII. "Urea Solution." 



1. To bouillon prepared as in I. add 1% urea and boil 

 until dissolved. 



2. Sterilize in the autoclave as usual. 



XIX. "Urea Agar." 



1. Make a paste of 20 gms. of powdered agar in cold 

 water and add to 1,000 cc. of "Urea Solution." 



2. Boil until dissolved. 



3. Filter through absorbent cotton and sterilize as usual. 



