80 LiBOEATORY BACTERIOLOGY. 



XXVII. ''Solution for Sulfat Reduction." 



1. Dissolve in 1,000 cc. of tap water : 



0.5 gm. KjHPO.. 

 5.0 gms. sodium lactate. 

 1.0 gm. asparagin. 

 1.0 gm. MgSOi. 

 Trace ferrous sulfat. 



2. Sterilize in the autoclave as usual. 



XX VIII. Sugar Broths. 



1. 1 gm. of any of the sugars (dextrose, maltose, saccha- 

 rose, mannite, or lactose) is added to 100 cc. of bouillon. 



2. Fermentation tubes are filled and sterilized in the steam 

 sterilizer for 30 minutes on three consecutive days. 



XXIX. DunJiam's Solution. 



1. 



1,000 cc. distilled water. 

 10 gms. Witte's peptone. 

 5 gms. NaCl. 



2. Fill into test tubes and sterilize in the autoclave as 

 usual. 



