Source. 



. Dnte ot Isolation Name . 



.Croup No. (•) . 



Agar 

 Hanging-Block 



DETAILED FEATURES. 



NOTE — Underscore required terms. Observe 

 notes and glossary of terms on opposite side of 

 card. 



I. MORPHOLOGY (=) 



1. Vegetative Cells, Medium usedi 



temp age days 



Form, rounds short rods, long rods, short 



chains^ long chains, 'filaments, commas, 



short spirals, long spirals, Clostridium, 



cuneate, clavate, curved. 



Limits of Size 



Size of Majority 



Ends, rounded, truncate, concave. 



Orientation (grouping) 



Chains (No. of elements) 



t^hort Chuiiis, lontj ehain.'<. 

 Orientation ot Cliains, parallel, 

 irretiuUir. * 



Aj 2. Sporangia, medium used temp 



age days 



Form, elliptical, short rods, spindled, clavate, 



drumsticks. 

 Limits of Size Size of Majority 



{Orientation (grouping) 

 Ciuxins (No. of elements) 

 Orientation of Cbains, parallel, 

 irregular. 



Location of Endosporos, central, polar. 

 3. Endospores. 



Form, 7'ound, elliptieal, elongated. 



Limits of Size 



Size of Majority 



Wall, thicK, thin. 



Sporangium wall, adherent, not adherent. 

 Germination, equatorial, oblique, polar, bi- 

 polar, bit strelehing. 



j4. Flagella No Attachment polar, bipolar, 



-'■* peritrichiatc. How Stained 



5. Capsules, present on 



6. Zoogloea, Pseudozoogloea. 



7. Involution Forms, on . . . .in. . .days at...°C. 



8. Staining Reactions. 



1 :10 watery fuchsia, gentian violet, carbol 



fuchsin. 

 Loelller's alltaliue methylene blue. 

 Special Staius. 



Gram Glycogen 



Fat Acid fast 



Neisser 



II. CULTURAL FEATURES ( = ) 



■i 1. Agar Strolie. 



Growth, invisible, scanty, moderate, abundant. 

 Form of growth, filitorni, echinulate, beaded, 



spreading, plumose, arborescent, rhixoid. 

 Elevation of growth, flat, effuse, raised, con- 



Lustre, glistening, dull, cretaceous. 



Topograpliy, smooth, contoured, rugose, ver- 

 ru^ose. 



Optical Characters, opaque, translucent, opal- 

 escent, iridescent. 



Chromogenesis (*) 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, mem- 

 branous, coriaceous, brittle. 



Medium grayed, browned, reddened, blued, 

 greened. 



Potato. 



Growth scanty, moderate, abundant, tran- 

 sient, persistent. 

 Form of growth, filiform, echinulate, beaded, 

 spreading, plumose, arborescent, rhizoid. 



Elevation of growth, flat, effuse, raised, con- 

 ve.T. 



Lustre, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, ver- 

 rueose. 



Chromogenesis (") Pigment in water 



insoluble, soluble; other solvents 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, mem- 

 branous, coriaceous, brittle. 



Medium grayed, browned, reddened, blued, 

 greened. 

 3. LoetDer's Blood Serum. 



Stroke invisible, scanty, moderate, ahundant. 



Form of growth, filiform, echinulate, beaded, 

 spreading, plumose, arborescent, rhisioid. 



Elevation of growth, fiat, effuse, raised, con- 

 vex. 



Lustre, glistening, dull, cretaceous. 



Topography, smooth, contoured, rugose, ver- 

 rueose. 



Chromogenesis (*) 



Medium grayed, browned, reddened, blued, 

 greened. 



Liquefaction begins in...d, complete in...d. 

 '^ 4. Agar Stab. 



Growtli uniform, best at top, best at bottom; 

 surface growth scanty, abundant; re- 

 stricted, wide-spread. 



Line of puncture, filiform, beaded, papillate, 

 villous, plumose, arborescent; Uquefaotion. 



vi .r, 



^2, 



<7. 



^ 10. 



11. 



Gelatin Stab. 



Growth uniform, hest at top, best at bottom. 



Line of puncture, filiform, beaded, papillate, 

 villous, plumose, arborescent. 



Liquefaction, crateriform, napiform, infundi- 

 buliform, saccate, stratiform; begins in 

 d, complete in d. 



Medium fluorescent, broumed 



Nutrient Broth. 



Surface growth, ring, pellicle, flocculent, 

 iuembrunous, none. 



Clouding slight, moderate, strong; transient, 

 persistent ; none; fluid turbid. 



OdoT, absent, d^ecided, resembling 



Sediment, compact, flocculent, granular, flaky, 

 viscid on agitation, abundant, scant. 



Milk. 



Clearing without coagulation. 

 • Coagulation prompt, delayed, absent. 



Extrusion ot whey begins in days. 



Coaglum slowly peptonized, rapidly pepton- 

 ized. 



Peptonization begins on d, completed on 



d. 



Reaction, Id . . . , 2d . . . , 4d . . . , lOd . . . , 20d . . . 



Consistency, slimy, viscid, unchanged. 



Medium browned, reddened, blued, greened. 



Lab ferment, present, absent. 



Litmus Milk. 



Acid, alkaline, acid then alkaline, no change. 



Prompt reduction, no reduction, partial slow 

 reduction. 



Gelatin Colonies. 



Growtli slow, rapid. 



Form, punetifonn. round, irregular, ameboid, 



myeelloiil. filiimintous, rhizoid. 

 Ifllevattou, flat, effuse, raisiil, convex, pulvi- 



nalc, cnilcrifdnii {liquefying). 

 Edge, entire, undulate, lobatc, erase, lacerate. 



fimhriiilc, filamentous, fioccose, curled. 

 Liquefaction, cup, saucer, spreading. 

 Agar Colonies. 



Growth slow, rapid, (temperature ) 



Form, punetiforni. round, irregular, ameboid, 



miiccliuiti, lilauK ntous. rhizoid. 

 Surface siuimth, rough, concentrically ringed, 



railiatc, striate. 

 Elevation, flat, effuse, raised, convex, pulvi- 



nate, utiibunatc. 

 Edge, entire, undulate, lobate, erase, lacerate, 



fluibriate, floccose, curled. 

 Internal structure, amorphous, finely-, 



coarsely-, granular, grmnose, fllamentous, 



fioccose, curled. 

 Starch Jelly. 

 Growth, scanty, copious. 

 Diastasic action, absent, feeble, profound. 



Medium stained 



Silicate Jelly (Fermi's Solution). 

 Growth copious, scanty, absent. 



Medium stained 



Cohn's Solution. 



tJrowtn copfoBs, scattty, aVsenf:~ 



Medium fluorescent, non-fluorescent. 



Uschinsky's Solution. 



Growth copious, scanty, absent. 



Fluid riscid, not viscid. 



Sodium Chloride in Bouillon. 



I'er cent, inhibiting growth 



(irowth in Bouillon over Chloroform, unre- 

 strained, feeble, absent. 

 Nitrogen. Obtained from peptone, asparagin, 



glycocoll, urea, ammonia salts, nitrogen. 

 Best media for long-continued growth 



m. Quick tests for differential purposes. 



111. PHYSICAL AND BIOCHEMICAL 

 URBS. 



2. Ammonia production, feeble, moderate, strong, 



absent, masked by acids. 



3. Nitrates in nitrate broth, 

 Reduced, not reduced. 



Presence of nitrites ammonia 



" " nitrates free nitrogen 



4. Indol production, feeble, moderate, strong. 



0. Toleration of Acids : Great, medium, slight. 

 Acids tested 



6. Toleration of Na O H : great, medium, slight. 



7. Optimum reaction for growth in bouillon, 



stated in terms of Fuller's scale 



8. Vitality on culture media : brief, moderate, 



long. 

 0. Temperature relJttions ; 



Thermal death-point (10 minutes exposure 

 in nutrient brotli when this is adapted to 

 growth of organism) C. 



Optimum temperature for growth C. : 



or best growth at 15° C, 20° C, 25° C, 

 30° C, 37° C, 40° C, 50° C, 60° C. 



Maximum temperature for growth C. 



Minimum temperature for growth C. 



10. Killed readily by drying : resistant to drying. 



11. Per cent, killed by freezing (salt and crushed 



ice or liquid air) 



12. Sunlight : Exposure on ice in thinly sown 



agar plates: one-half plate covered (time 

 15 minutes), sensitive, not sensitive. 

 Per cent, killed 



13. Acids produced 



14. Alkalies produced 



15. Alcohols 



10. Ferments ; pepsin, trypsin, diastase, inver- 

 tase, pectase, cytase, tyrosinase, oxidase, 

 peroxidase, lipase, catalasc, glucose, galac- 

 tose, lab, etc. 



IT. Crystals formed : 

 18. Effect of germicides : 



Method u.sed. 



FEAT- — 



« 9 



O M 



iar 



r' 



IV. PATHOGENICITY. 



1. Pathogenic to Animals : 



Insects, crustaceans, fishes, reptiles, birds, 

 mice, rats, guinea pigs, rabbits, dogs, cats, 

 slieep, goats, cattle, horses, monkeys, 

 man 



2. Pathogenic plants : 



3. Toxins, soluble, endotoxins. 



4. Non-toxin forming. 



5. Immunity bactericidal. 



0. Immunity non-bactericidal. 

 7. Loss of virulence on culture media : prompt, 

 gradual, not observed in months. 



BRIEF CHARACTERIZATION. 

 Mark + or O, and when two 

 terms occur on a line erase the 

 one which does not apply unless 

 both apply. 



