44 LABORATORY BACTERIOLOGY 



employed in studying and differentiating species of bacfferia 

 have been arranged arbitrarily in groups. Large test tubes 

 containing agar and gelatin for making plate cultures are not 

 included in these groups. 



Group A. Media commonly used. 



A tube of bouillon. 



A tube of sugar-free bouillon. 



A tube of agar. 



A tube of gelatin. 



A tube of milk. 



A tube of litmus milk. 



A tube of potato. 



Group B. Media favorable to determine the power of bac- 

 teria to ferment the sugars with the formation of acids. 



A tube of bouillon containing i % grape sugar (dextrose, 



glucose). 

 A tube of bouillon containing i % milk sugar (lactose). 

 A tube of bouillon containing i % cane sugar (saccharose). 



Group C. Media favorable for determining the production 

 of gas. 



A tube of agar containing i % grape sugar. 

 ,■ A tube of agar containing i % milk sugar. 

 / A tube of agar containing i % cane sugar. 



Group D. Media and tubes favorable for approximate gas 

 analysis and the determining of the aerobic or anaerobic 

 tendencies. 



Fermentation tube containing i % grape-sugar bouillon. 

 Fermentation tube containing i % milk-sugar bouillon. 

 Fermentation tube containing i % cane-sugar bouillon. 



The fermentation tubes containing bouillon with sugars may be 

 substituted for the media containing sugars in Groups B and C if 

 desired. 



