8o LABORATORY BACTERIOLOGY 



The Johns Hop. Hosp. Reports, Vol. V, p. 343. Smith, The Jour, 

 of the Boston Soc. of Med. Sciences, June, 1898. Hiss, The 

 Jour, of Exp. Med., Vol. II, 1887, p. 677. 



120. Work for this exercise. Examine the plate cultures 

 made from the intestine for the colon bacillus. 



Determine the approximate number of colonies on each 

 plate and note especially the number of colonies of B. colt 

 communis and describe their appearance. 



Inoculate a tube of agar, one of milk, and a fermentation 

 tube of glucose bouillon from one of the colonies. Study 

 these cultures in the next exercise and compare them with 

 the notes on cultures of B. coli commu?iis in these media. 



Examine and carefully describe the cultures of B. cholera 

 suis and B. typhosus. Note especially the reaction of the cul- 

 tures in the fermentation tubes. Examine the bouillon cul- 

 tures microscopically in hanging-drop and in stained cover- 

 glass preparations. Describe the appearance of the bacteria. 



Make a series of 3 gelatin plate cultures from the bouillon 

 culture of each organism. 



