90 LABORATORY BACTERIOLOGY 



EXERCISE XXXV 



BACTERIUM SEPTIC^MI^ HEMORRHAGICA OR 

 MICROCOCCUS LANCEOLATUS {continued) 



131. Work for this exercise. Reexamine all the cultures 

 of these bacteria, paying special attention to the reactions of 

 the liquid cultures. 



Make the indol test with the cultures in sugar-free bouillon. 



Make, stain, and examine a cover-glass preparation from an 

 organ and the blood of a rabbit which has died from the inocu- 

 lation with swine-plague bacteria or with Micrococcus lanceo- 

 latus (the rabbit will be furnished by the instructor). Stain 

 the preparations with an aqueous solution of fuchsin. Study 

 the bacteria in these preparations and carefully compare the 

 two. Indicate in the notes the differences, if any are found. 



Give in the notes a list of characters and cultural properties 

 of Bad. septicemia hemorrhagiccR and of M. lanceolatus that 

 are of differential value. In what properties do they differ 

 from B. cholera suis and B. typhosus. 



Keep the cultures until the next exercise and compare them 

 again, after which they may be rejected. 



