94 LABORATORY BACTERIOLOGY 



EXERCISE XXXVII 



BACTERIUM MALLEI 



136. This organism grows most characteristically on potato 

 and somewhat feebly in the other media heretofore used. It 

 develops readily on acid agar and in acid glycerin agar and 

 acid glycerin bouillon. For this reason it is not inoculated 

 into all of the media. In diagnosing glanders it is customary 

 to inoculate guinea pigs with the suspected material (see 

 Appendix III). From the lesions in these animals, if the dis- 

 ease develops, pure cultures can usually be obtained. It can 

 be identified by its morphologic and cultural characters. 



References. Chapters on Bacterium mallei in text-books. 

 Smith, The Jour, of Comp. Med. and Vet. Archives, March, 1890. 

 De Schweinitz and Dorset, Jour, of the Am. Chem. Soc, Vol. 

 XVII, 1898. Finkelstein, Centralb. f. Bakteriologie u. Parasi- 

 tenkunde, Bd. XI, 1892, S. 433. Frothingham, Proceedings Am. 

 Vet. Med. Asso., 1901, p. 360 (Straus' method of diagnosing 

 glanders). See recent text-books. 



137. Work for this exercise. Inoculate a tube of potato, 

 one of agar, one of acid agar, one of acid glycerin agar, one 

 of glucose agar, one of bouillon, and one of acid glycerin 

 bouillon from a culture furnished. (The special media here 

 introduced will be furnished by the instructor.) 



Stain cover-glass preparations (furnished) made from the 

 lesions in guinea pigs which were inoculated with this organ- 

 ism. Stain one with alkahne methylene blue and one with car- 

 bol fuchsin. Note especially the morphology and the extent 

 to which the organisms take the stain. 



Reexamine the cultures of avian tubercle and complete the 

 notes on the same. 



