BACTERIUM MALLEI 95 



EXERCISE XXXVIII 



BACTERIUM MALLEI {continued) 



138. Work for this exercise. Examine and carefully describe 

 all the cultures of Bacterium mallei. 



Make 2 cover-glass preparations from the acid agar and 

 from the bouillon cultures, and stain one of each with alkaline 

 methylene blue and one with carbol fuchsin. Describe the 

 bacteria and make a drawing of a few of them. Preserve i 

 preparation. Keep the cultures and reexamine them at each 

 of the 3 following exercises. Note especially the character 

 and color of the growth on the potato and in the acid glycerin 

 bouillon. 



Examine carefully the lesions produced by the inoculation 

 of Bacterium mallei in a male guinea pig (Straus' method 

 of diagnosing glanders). 



Inoculate by Liborius' method (§ 141) 2 tubes of liquid agar, 

 one sugar free, the other containing glucose, 2 fermentation 

 tubes, one sugar free, the other containing i % glucose bouillon 

 from a culture of an anaerobic bacillus, the bacillus of malig- 

 nant oedema or of symptomatic anthrax is preferable (fur- 

 nished), and place the inoculated tubes in the incubator. 



