BACTERIUM ANTHRACIS lOI 



EXERCISE XLII 



BACTERIUM ANTHRACIS icontimied) 



148. Work for this exercise. Reexamine all of the cultures 

 of Bacteriutn anthracis and describe any changes in their 

 appearance which may have taken place. 



Examine the agar culture for spores in a hanging-drop prep- 

 aration and in a stained cover-glass preparation. Describe 

 the appearance of the bacteria and spores in a preparation 

 from each. 



Study and describe the appearance of the colonies on the 

 agar plates. Make an outline drawing of a few of the surface 

 and deep colonies. 



Reject all cultures except the agar plates, which may be 

 kept until the next exercise for further observation before 

 rejecting. (These cultures should be put in charge of the 

 instructor, who will see that the spores are destroyed before 

 the tubes are cleaned.) 



Inoculate a tube of each medium in Groups A and B and 

 also a tube of LoefHer's blood serum and glycerin agar with 

 Bacterium diphthericB from a culture furnished for study at the 

 next exercise. 



References. Chapters on anthrax in recent editions of text- 

 books. Chester, Report Delaware College Agric, Exp. Station, 

 July, 1895. Moore, Report N. Y. State Com. of Agr., 1897. 

 Emmerich and Saida, Centralb. f. Bakter. u. Parasitenkunde, Bd. 

 XXVII, S. 776. Bongert, Ibid., Bd. XXXIV, S. 497. Preisz, Ibid., 

 Bd. XXXV, S. 280. 



