CONTElifTS. 



INTRODUCTION. 



PAGE3 



"Omne vivum ex vivo" — The overthrow of the doctrine of 

 spontaneous generation — Earlier bacteriological studies — 

 The birth of modern bacteriology 17-30 



CHAPTER I. 

 Definition of bacteria — Their place in nature — Difference be- 

 tween parasites and saprophytes — Nutrition of bacteria — 

 Products of bacteria — Their relation to oxygen — Influence 

 of temperature upon their growth 31-45 



CHAPTER II. 

 Morphology of bacteria — Grouping — Mode of multiplication — 

 Spore-formation — Motility . 46-58 



CHAPTER III. 

 Principles of sterilization by heat — Methods employed — Dis- 

 continued sterilization — Sterilization under pressure — Ap- 

 paratus employed — Chemical disinfection and sterilization . 59-86 



CHAPTER IV. 



Principles involved in the methods of isolation of bacteria in 

 pure culture by the plate method of Koch — Materials em- 

 ployed 87-93 



CHAPTER V. 



Preparation of nutrient media — Bouillon, gelatin, agar-agar, 



potato, blood-serum, etc 94-125 



vii 



