Till CONTENTS. 



CHAPTER VI. 



PAGES 



Preparation of the tubes, flasks, etc., in which the media are 

 to be preserved 126-129 



CHAPTER VII. 

 Technique of making plates — Esmarch tubes, Petri plates, etc. 130-141 



CHAPTER. Vm. 



The incubating-oven — Gas-pressure regulator — Thermo-regu- 

 lator — Safety burner employed in heating the incubator . . 142-149 



CHAPTER IX. 



The study of colonies — Their naked-eye peculiarities and 

 their appearance under different conditions — Differences 

 in the structure of colonies from different species of bac- 

 teria — Stab-cultures — Slant-cultures 150-156 



CHAPTER X. 



Methods of staining — Solutions employed — Preparation and 

 staining of cover-slips — Preparation of tissues for section- 

 cutting — Staining of tissues — Special staining-methods . 157-197 



CHAPTER XI. 



Systematic study of an organism — Points to be considered iu 

 identifying an organism as a definite species . . . 198-229 



CHAPTER XII. 



Inoculation of animals — ^ Subcutaneous inoculation — Intra, 

 venous injection — Inoculation into the great serous cavities 

 and into the anterior chamber of the eye — Observation of 

 animals after inoculation . . 230-251 



CHAPTER XIII. 



Post-mortem examination of animals — Bacteriological exami- 

 nation of the tissues — Disposal of tissues and disinfection 

 of instruments after the examination — Study of tissues and 

 exudates during life ... 252-260 



