228 SAOTEMOLOGY. 



8. The character of its growth in fluid media, as in 

 bouillon, milk, litmus-milk, rosolic-acid -peptone solu- 

 tion, and in bouillon containing glucose. 



9. Does it grow best in acid, alkaline, or neutral 

 media ? 



10. Is the normal reaction of the medium altered by 

 its growth ? Is its growth accompanied by the produc- 

 tion of indol ? Is the indol associated with the coinci- 

 dent production of nitrites? 



11. Is its growth accompanied by the production of 

 gas, as evidenced by the appearance of bubbles in the 

 media — both in media containing fermentable sugars 

 and those from which these bodies are absent ? When 

 cultivated in sugar-bouillon in the fermentation-tube, 

 what proportion of gas is evolved under known condi- 

 tions ? How much of this gas is carbonic acid, and how 

 much is explosive? 



12. At what temperature does it thrive best, and 

 what are the lowest and highest temperatures at which 

 it will develop? What is its thermal death- point, both 

 by steam and dry-air methods of determining this point? 



13. What is its behavior when exposed to chemical 

 disinfectants and antiseptics? Does it withstand dry- 

 ing and other injurious influences, both in the vegeta- 

 tive and spore stages ? The germicidal activity of the 

 blood-serum of difierent animals may also be tried 

 upon it. 



14. Its pathogenic powers — modes of inoculation by 

 which these are demonstrated ; quantity of material used 

 in inoculation ; duration of the disease and its symp- 

 toms ; lesions produced, and distribution of the bacteria 

 in the inoculated animals ; which animals are susceptible 

 and Avhich immune, and the character of its pathogenic 



