234 BACTERIOLOOY. 



with its head toward the perforated bottom. It is then 

 easily possible to make subcutaneous inoculation by 

 taking up a bit of skin through the slit in the side of 

 the box, or to make intraperitoneal injection by drawing 

 the posterior extremities slightly from the box and 

 holding them steady between the index and second 

 finger, as seen in Fig. 45. It is also very convenient 

 for use Avhen the rectal temperature of these small ani- 

 mals is to be taken. The manipulations can easily be 

 made without the aid of an assistant. Its construction 

 is seen in Fig. 45.^ 



For ordinary subcutaneous inoculations at the root 

 of the tail in mice a simple apparatus consists of a 

 piece of board about 7 x 10 cm. and 2 cm. thick, 

 upon which is tacked a hollow truncated cone of wire 

 gauze, about 6 cm. long and about 1.5 cm. in diameter 

 at one end and 2 cm. at its other end. This is tacked 



Fig. 46. 



Mouse-holder, with mouse in proper position. 



upon the board in such a position that its long axis is 

 in the long axis of the board, being equidistant from its 

 sides. Its small end is placed at the edge of the board. 

 The mouse is taken up by the tail by means of a pair 



' Centralblatt fiir Bakteriologie und Parasitenkunde, 1895, Bd. xviii. 

 p. 530, 



