Q0N0O0C0V8. 293 



gree of heat that apparently interferes with the nutri- 

 tive value of the serum contained in the medium. 



AVhen inoculated with gonorrhoeal pus, by smearing 

 a loopful over the surface, the tubes are to be kept at 

 from 37° to 38° C. The organism does not develop 

 properly at a temperature below this point. 



After twenty-four hours the colonies of the gono- 

 coccus appear on the surface of the medium, accord- 

 ing to Wright, as very tiny, grayish, semi-translucent 

 points. After forty-eight hours they may be about 

 1 millimetre or so in diameter, slightly elevated, with 

 a rounded outline, grayish in color, and semi-translu- 

 cent by transmitted light. By reflected light their sur- 

 face has the appearance of frosted glass. Later, if few 

 in number, so that their growth is unimpeded, the colo- 

 nies may attain a diameter of 2 millimetres or more, be- 

 come thicker and denser, with a faintly brownish tinge 

 about their centres, and a slightly irregular outline. 



Under a low power of the microscope a fully de- 

 veloped colony is seen to consist of a general circular 

 expansion, with thin, translucent, smooth, sharply de- 

 fined margin, but becoming brownish, granular, and 

 thicker toward the central portion, which is made up 

 of coarse, granular, brown-colored clumps closely packed 

 together. 



The appearances coincide with the figure of such a 

 colony given by Wertheim.* 



Wassermann^ calls attention to the success he has 

 had in cultivating this organism upon a mixture of 

 swine-serum and nitrose, the latter being a com- 



' Deutsche med. Woohenschrift, 1891, No. 50 ; Centralblatt fiir Gyna- 

 kologie, 1891, No. 24. 

 2 Zeitschrlft fiir Hygiene und Infektionskrankheiten, Bd. xvii. p. 298. 



