BACILLUS PYOCYANEUS. 309 



of tbe enzymes encountered in the animal body ? 

 Which? and Why? 



Inocui^ation into Animals. — As a rule, cultures 

 of this organism obtained directly from the discharges 

 of the wound are caj)able, when introduced into ani- 

 mals, of producing diseased conditions ; but cultures 

 kept on artificial media for a long time may in part, or 

 completely, lose this power. 



When guinea-pigs or rabbits are inoculated subcuta- 

 neously with 1 c.c. of virulent fluid cultures of this 

 organism, death usually results in from eighteen to 

 thirty-six hours. At the seat of inoculation there are 

 found an extensive purulent infiltration of the tissues 

 and a marked zone of inflammatory oedema. 



When introduced directly into the peritoneal cavity 

 the results are also fatal, and at autopsy a genuine 

 fibrinous peritonitis is found. There is usually an ac- 

 cumulation of serum in both the peritoneal and pleural 

 cavities. At autopsies after both methods of inocula- 

 tion the organisms will be found in pure cultures in 

 the blood and internal viscera. 



When animals are inoculated with small doses (less 

 than 1 c.c. of a bouillon culture) of this organism death 

 may not ensue, and only a local inflammatory reaction 

 (abscess-formation) may be set up. In these cases the 

 animals are usually protected against subsequent inocu- 

 lation with doses that would otherwise prove fatal. 



Most interesting in connection with bacillus pyooy- 

 aneus is the fact, as brought out in the experiments of 

 Bouchard, and of Charrin and others, that its products 

 possess the power of counteracting the pathogenic ac- 

 tivities of badllus anthrads. That is to say, if an 

 aninwl be inoculated with a virulent anthrax culture, 



