352 BACTERIOLOGY. 



eifect, aud others cause lesions of more or less impor- 

 tance, the result being dependent upon the quantity em- 

 ployed and the mode of inoculation. ,By subcutaneous 

 or intraperitoneal injection of pure cultures the result is 

 usually negative. Occasionally the superficial lymphatic 

 glands in the neighborhood of the site of inocijlation 

 may be inflamed and purulent. This we have seen only 

 with the subcutaneous inoculation. If pure cultures be 

 injected into the peritoneal cavity along with some 

 sterile, irritating substance, such as sterilized butter, a 

 widespread fibrinopurulent peritonitis is commonly the 

 result. 



When injected directly into the circulation of rabbits, 

 the kidneys are almost uniformly affected, and in the 

 majority of instances they are, singularly enough, the 

 only organs in which lesions are to be detected. If, for 

 instance, a cubic centimetre of a carefully prepared 

 suspension in bouillon of, let us say, Moller's grass 

 bacillus II., be injected into the circulation of a rabbit, 

 and the animal be killed after twelve to fourteen days, 

 the kidneys will be found marked by gray or yellowish 

 points that range in size from that of a pin-point to 

 that of a pin-head. They are sometimes very few in 

 number, but in other cases both kidneys may be thickly 

 studded with them. Often they are not elevated above 

 the cortex of the organ, but in as many cases they are 

 sharply defined, yellow in color, and stand up promi- 

 nently from the cortical surface, being at the same time 

 so adlierent to the capsule that the removal of the latter 

 tears them out bodily from the substance of the organ. 

 In the very early stages of development these nodules 

 are often difficult to distin£!;uish from young tubercles, the 

 reaction of the tissues being, as in the case of tubercles, 



