THE BACILLUS OF GLANDERS. 381 



hours when exposed to 50° C, and in five minutes by 

 55° C. 



It grows both with and without oxygen ; it is there- 

 fore facultative as regards its relation to this gas. 



On cover-slips it stains readily ^^'ith all the basic 

 aniline dyes, and, as a rule, as stated, presents conspic- 

 uous irregularities in the way that it takes up the dyes, 

 being usually marked by deeply stained areas that alter- 

 nate with points at which it either does not stain at all 

 or only slightly. 



The animals susceptible to infection by this organism 

 are horses, asses, field-mice, guinea-pigs, and cats. 

 Baumgarten records cases of infection in lions and 

 tigers that were fed, in menageries, with flesh from 

 horses affected with the disease. Rabbits are but 

 slightly susceptible ; dogs and sheep still less so. Man 

 is susceptible, and infection not rarely terminates fatally. 

 White mice, common gray house-mice, rats, cattle, and 

 hogs are insusceptible. 



Inoculation Expeeiments. — The most favorable 

 animal upon which to study the pathogenic properties 

 of this organism in the laboratory is the common field- 

 mouse. When inoculated subcutaneously with a small 

 portion of a pure culture of the glanders bacillus death 

 ensues in about sevent}'--two hours. The most conspicu- 

 ous tissue-changes will be enlargement of the spleen, 

 which is at the same time, almost constantly, studded 

 with minute gray nodules, the typical glanders nodule. 

 They are rarely present in the lungs, but may frequently 

 be seen in the liver. From these nodules the glanders 

 bacillus may be obtained in pure culture. With the 

 exception of the characteristic nodules, the disease as 

 seen in this animal presents none of the features that 



