STAINING IN TISSUES. 383 



purpose, though perhaps no better than some of the 

 others, are as follows : 



a. Transfer the sections from alcohol to distilled 

 water. This lessens the intensity with which the stain 

 subsequently takes hold of the tissues, by diminish- 

 ing the activity of the diffusion that would occur if 

 they were placed from alcohol into watery solutions of 

 the dyes. Transfer from distilled water to the slide, 

 absorb all water with blotting-paper, and stain with 

 two or three drops of 



Carbol-fuchsin . . ... ... . 10 c.c. 



Distilled water ...... . 100 c.c. 



for thirty minutes; absorb all superfluous stain with 

 blotting-paper, and wash the section three times with 

 0.3 per cent, acetic acid, not allowing the acid to act 

 for more than ten seconds each time. Remove all 

 acid from the section by carefully washing in distilled 

 water ; absorb all water by gentle pressure with blot- 

 ting-paper ; and finally, at very moderate heat, or with 

 a small bellows (Kiihne), dry the section completely on 

 the slide. When dried clear in xylol and mount in 

 xylol balsam. 



b. Transfer the sections from alcohol to distilled 

 water ; from water to the dilute fuchsin solution, and 

 gently warm (about 50° C.) for fifteen to twenty 

 minutes. Transfer sections from the staining-solution 

 to the slide, absorb all superfluous stain with blotting- 

 paper, and then treat them with 1 per cent, acetic acid 

 from one-half to three-quarters of a minute. Remove 

 all trace of acid with distilled water, absorb all water by 

 gentle pressure with blotting-paper, and then treat the 

 sections with absolute alcohol by allowing it to flow 



