PSE UD 0-DIPHTHERITIG BA GILL US. 



403 



develop at a temperature not lower than 34° C. and not 

 higher than 36° C. ; and it should be not younger than nine 

 and not older than twenty-four hours. A cover-glass prep- 

 aration made from such a culture is stained as follows : 



a. It is subjected to the following mixture for from 

 one to three seconds : 



Methylene-blue (Griibler's) 1 gramme. 



Alcohol (96 per cent.) 20 c.c. 



When dissolved, mix with 



Acetic acid . . 50 c.c. 



Distilled water . . . 950 c.c. 



b. After thoroughly rinsing in water, it is stained for 

 from three to five seconds in vesuvin (Bismarck-brown), 



Fig. 70. 



BacUlue diphtkerise, stained by Neisser's method. 



2 grammes, dissolved in 1 litre of boiling distilled water, 

 filtered, and allowed to cool. It is again rinsed in water 



