SPIRILLUM CHOLERA ASIATIC JE. 



451 



naturally grow to a larger size than do those upon 

 gelatin at 22° C. 



In stab-cultures in gelatin there appears at the top 

 of the needle-track after thirty-six to forty-eight hours 

 at 22° C. a small, funnel-shaped depression. As the 

 growth progresses liquefaction occurs about this point. 

 In the centre of the depression can be distinguished 



Fig. 78. 



^i gprS ji 



a b e d 



Stab-culture of the spirillum of Asiatic cholera in gelatin, 

 at 18° to 20° C. 

 a. After twenty-four hours, b. After forty-eight hours, c. After seventy- 

 two hours, d. After ninety-six hours. 



a small, dense, whitish clump, the colony itself. As 

 growth continues the depression increases in extent 

 and ultimately assumes an appearance that consists in 



