MILLER'S SPIRILLUM. 481 



brownish-yellow growth appears that is surrounded by a 

 drier, colorless zone. It is not lobulated. 



In milk containing neutral litmus tincture a pink color 

 appears after two to three days at 37° C. ; after four 

 days the milk is almost decolorized and coagulation of 

 the casein is beginning, with a layer of clear Avhey above 

 it. During the subsequent twenty-four hours complete 

 separation of the contents of the tube into clot and whey 

 takes place. 



In Dunham's peptone solution it does not form indol, 

 and the reaction for this body does not appear with 

 either sulphuric acid alone or plus sodium nitrite. 



It causes liquefaction of both coagulated blood-serum 

 and egg-albumin. 



There is no pellicle formed as a result of its growth 

 in bouillon. 



It does not produce fermentation of glucose. 



In rosolic-acid-peptone solution its growth causes in- 

 tensification of the red color in four or five days at 

 37° C. 



By Koch's method of introducing cultures into the 

 stomach of a guinea-pig this organism produced the 

 death of three out of fifteen animals experimented upon 

 • — the deaths being due most probably to the toxic action 

 of the products of growth that were introduced with the 

 organisms rather than to any pathogenic powers possessed 

 by the organism itself. 



miller's spirillum. 



Another spirillum that has been likened to that of 

 Koch is the one obtained by Miller from a carious tooth. 

 It has so many characteristics in common with the or- 

 ganism of Tinkler and Prior that Miller was inclined 

 dl 



