MILLER'S SPIRILLUM. 483 



at the centre than at the periphery, and the dense centre 

 is not sharply circumscribed, but fades oif into what has 

 the appearance of a delicate film. (See b, Fig. 86.) As 



lIG 8b 



6 



Colonies of Miller's spirillum on gelatin, at 20° to 22° C. x about 57 



diameters. 



II. Colony just beneath the surface of the gelatin. 6. Colony on the surface 



of the gelatin. 



the colonies become older they are sometimes marked 

 by irregular radii extending from periphery to centre, 

 like the spokes of a wheel. 



In stab-cultures in gelatin it rapidly produces lique- 

 faction, both at the surface and along the needle-track, 

 and in most respects gives rise to a condition very like 

 that resulting from the growth of Tinkler and Prior's 

 spirillum, though differing from it in certain details. 

 (See a, b, c, d, Fig. 87.) 



On agar-agar nothing of special interest appears as a 

 result of its development. 



On potato its growth is very like that of the cholera 

 spirillum, viz., it appears at 37° C. as a dry, white patch 

 that lies quite flat upon the surface and can often only 

 be seen when the tube is held to the light at a particu- 

 lar angle. 



Its growth in bouillon is not characteristic. It does 

 not form a pellicle. 



It causes liquefaction of both coagulated blood-serura 

 and egg-albumin. 



