502 BAOTEBTOLOGT. 



such as sheep or cattle, are to be protected by vaccina- 

 tion with these vaccines, a subcutaneous inoculation of 

 about 0.3 c.c. of the first vaccine is usually given. 

 This should be practically without noticeable effect, 

 causing neither rise of body-temj)erature nor other 

 constitutional or local symptoms. After a period of 

 about two weeks the second vaccine is injected in the 

 same way ; this may or may not cause disturbance. 

 In the event of its doing so the symptoms are rarely 

 alarming, and, if the vaccines have been properly pre- 

 pared and tested before use, they disappear within a 

 short time after the injection. 



In the large majority of cases sheep, bovines, horses, 

 and mules may be safely protected against anthrax by 

 the careful practice of this method. 



EXPERIMENTS. 



Prepare three cultures of anthrax bacilli — one upon 

 gelatin, one upon agar-agar, and one upon potato. 

 Allow the gelatin culture to remain at the ordinary 

 temperature of the room, place the agar-agar culture 

 in the incubator, and the potato culture at a temper- 

 ature not above 18° to 20° C. Prepare cover-slips 

 from each from day to day. "What differences are 

 observed ? 



Prepare two potato cultures of the anthrax bacillus. 

 Place one in the incubator and maintain the other at a 

 temperature of from 18° to 20° C. Examine them 

 each day. Do they develop in the same way ? 



From a fresh culture of anthrax bacilli, in which 

 spore-formation is not yet begun (which is the surest 



