v] METHODS OF INOCULATION 87 



Buchner places the culture-tubes, after inoculation, 

 plugged simply with cotton wool but not sealed, into a glass 

 bottle, and then adds into this carefully and liberally 

 pyrogallic acid and liquor potassae (for each gramme of 

 pyrogallic acid i cc. of liquor potassae) and hermetically 

 closes the bottle. I have not found any advantage in 

 using other methods over the two just described, and I 

 use the second or Buchner's method to grow anaerobic 

 microbes on the slanting surface of solid media. 



