XI] BACILLI , SPECIFICALLY PATHOGENIC 229 



cholera (see chapter on Cholera). The colon variety ob- 

 tained from these cases of English cholera possesses, how- 

 ever, considerable virulence on the guinea-pig, producing in 

 this animal after subcutaneous injection of small or moderate 

 doses acute septicaemic infection and death. Of this 

 character appears to be the Bacillus neapolitanus isolated 

 by Emmerich from the intestinal fluid in cases of Asiatic 

 cholera. 



4. The aerobic bacillus of malignant cudema, which I 

 obtained from recently manured garden earth, produces 

 on subcutaneous inoculation into guinea-pigs and mice 

 extensive gangrene of the skin and muscle, with san- 

 guineous, malodorous exudation, and death in twenty-four 

 to thirty-six hours — a condition similar to that produced by 

 the anaerobic malignant oedema bacillus of Koch (also 

 obtainable from manured garden earth) ; in cultural respects, 

 in its motility and flagella, it is not distinguishable from 

 bacillus coli ; the chief difference from the latter consists in 

 the great virulence of the former. The subcutaneous 

 exudation as also the skin itself is crowded with the bacilli. 



5. A bacillus which in morphological and cultural 

 respects is closely related to the bacillus coli was found in 

 abundance in beef-pie {Portsmouth), which had caused in 

 those who partook of it severe gastro enteritic symptoms. 

 By feeding mice with the pie or with the broth cultures 

 of the bacillus acute gastro-enteritis was produced (Report 

 of the M.O. of the Loc. Gov. Board, 1890-91), and 

 thereby its difference from the bacillus coli was established, 

 for such a result is not to be obtained with the cultures of 

 the bacillus coli derived from the intestinal contents. 



6. A bacillus of which the cultural characters have not 

 been ascertained (it occurred at a time before solid culture 

 media were used), and of which therefore I am unable to 



