266 



MICRO-ORGANISMS AND DISEASE [chap. 



resemble in every respect the clumps seen in the bronchial 

 sputum. 



By staining a cover-glass film of the young colonies first 

 with rubin and afterwards with methyl-blue the sheath of 

 the threads is well differentiated as of pink colour from the 

 polar granules, or the rod-shaped protoplasm in the sheath. 



Fig ioo. — Film Specimen from a Broth Culture of Influenza Bacilli. 



.< lODO. 



The same result is obtained from the growth in broth, but 

 the most satisfactory specimens for microscopic observation 

 were obtained from Agar cultures. We possess specimens 

 from Agar cultures stained double as mentioned above, and 

 the rod-shaped character of the elements of considerable 

 portions of a thread is very strikingly marked by being 

 stained blue in the pink general sheath. 



On looking at the threads or clumps of any growth with 



