xiii] MICROBES OF MALIGNANT ANTHRAX 277 



spleen of an animal dead of anthrax, or with the bacillus or 

 spores of an artificial culture, die generally within forty eight 

 hours ; in some instances in twenty-four to thirty hours, 

 in other instances after forty-eight to sixty hours. The 

 blood in all instances contains the bacilli, the spleen is 

 large and full of bacilli, and so are the blood-vessels of most 

 other organs, the exudations, and the urine. In the placenta 

 of a pregnant guinea-pig dead in consequence of inoculated 

 anthrax, I have seen that the bacilli kept strictly as a rule 



Fig. io5. — Fro ,1 a Prep.\ratiom of Heart's Blood of a Glmnea-pk; dead 

 OF Anthrax. 



1. Red blood discs. 



2. White corpuscle. 



3. Bacilli anthracis, showing well their slieath. 

 Magnifying power 700. (Stained with Spiller's purplj.) 



within the maternal blood-vessels, and are wholly absent in 

 the blood of the vessels of the fcetus. Subcutaneous in- 

 oculation or injection into the cutis of minute quantities 

 of bacillus containing material (blood or virulent culture) 

 invariably produces death. Subcutaneous injection of 

 bacillus-containing material in the guinea-pig almost always 

 produces a characteristic oidcma, spreading sometimes over 

 a large area. The cedematous fluid is clear and contains 

 only a few bacilli. 



Any neutral or faintly alkaline material containing pro- 



