294 MICRO-ORGANISMS AND DISEASE [chap. 



which are equally numerous and virulent after passing 

 through several generations by inoculation." 



The disease often ends fatally in calves. 



The best method of staining the bacilli was found to be 

 this : The sections, both those prepared from the ulcerations 



Fig, 116. — Frosi a Section through Tongue of Calf, Ulcerative 

 Stomatitis. 



1. Muscular fibres. 



2. Inflamed tissue. 



3. Bundles of the bacilli. 



Magnifying power 700. (Stained with magenta.) 



of the calf's tongue and from the inoculated tissues of the 

 rabbit, are immersed in a mixture of magenta and methyl- 

 blue, then washed in spirit, and after clarifying in clove-oil 

 are mounted in Canada-balsam solution. The bacilli are 

 stained deep pink, the inflamed tissue blue. The bacilh 



