xni] MICROBES OF MALIGNANT ANTHRAX 305 



as also they appear to form longer rods and chains than at 

 first. The club-shaped forms and the chains of granules 

 and rods with spindle-shaped and clubbed ends also appear 

 sooner in the cultures ; these forms have nothing to do with 

 involution forms, as the)' can be demonstrated already in the 

 active and early phases of the development of the colonies. 



Fig. T22.— Film Stecimen or an Ar,,\R Culture oe Piacillus Dri'irrnERLE 



AETER A FEW DaYS' GrOWIII ; ClIAINS AND ClUCS AIvE WELL SHOWN. 



For the isolation of the diphtheria bacillus, serum (pure 

 blood-serum or, better, Loffler'sserum) ornutrient Agaris used, 

 for then the colonies if present can be recognised already after 

 twenty-four hours. As stated above, in some cases of mem- 

 branous dijjhtheria numerous colonies, occasionally in pure 

 culture, of the diphtheria bacilli can be easily obtained eitherby 

 rubbing a particle of the membrane over the slanting surface 



X 



