Xtii] MICROBES OF MALIGNANT ANTHRAX 319 



fifth and sixth days it was still soft, felt like oedema, and on 

 pressure a quantity of clear serum could be squeezed out 

 from it. After the death of the animals (one died after a 

 fortnight, the other was killed on the twenty-fifth day) the 

 tumour was examined, and it was found to be located in 

 the subcutaneous tissue, but was firmly connected both to 

 the skin above and the muscular tissue below, and was 

 surrounded by cedematous tissue. It was streaked white, 

 was firm, but on section clear serum could be pressed out 

 from it. Under the microscope the tissue of the tumour 

 was found to be of the same nature as diphtheritic material : 

 a general matrix of reticulated necrotic tissue in which rem- 

 nants of nuclei, outlines of blood-vessels, and remnants of 

 extravasated blood could be recognised ; this tissue shaded 

 gradually both into the cutis and into the surrounding muscles. 



Both animals showed normal temperature to the end, but 

 they both coughed and gradually fell off from feeding and 

 did not take any water. One of them by the end of the fort- 

 night suddenly became worse : it took no food or water, its 

 milk failed, its evacuations became scanty and dry, its breath- 

 ing became very rapid, and after a sudden collapse it died. 

 The other animal after twenty-four days (since inoculation) 

 grew much worse, and was therefore killed. 



In both animals the lymph glands nearest the left shoulder, 

 i.e. close to the tumour, were much enlarged, very cedema- 

 tous, and contained haemorrhage ; no change in the organs 

 of the throat ; both lungs showed extensive congestion, in 

 fact almost amounting to red hepatisation of the upper 

 lobes and the upper portion of the middle lobe, petechias, 

 and haemorrhagic patches under the pleura ; the pleural 

 lymphatics were everywhere in the congested portions con- 

 spicuous and distended, either with clear lymph, or, as was 

 the case in the second cow, tinged with blood. Cutting into 



