XIli] MICROBES OF MALIGNANT ANTHRAX 32I 



diphtheria bacilli introduced in the subcutaneous tissue pro- 

 duce here by growth and multiplication the chemical poison, 

 setting up the general disease in the viscera. The presence 

 of the diphtheria bacillus in the eruption of the cow could be 

 demonstrated both microscopically and by culture during 

 the vesicular and pustular stages ; in the latter also numerous 

 pus cocci. 



That in the cow the diphtheria bacillus as such passed into 

 the system of the animal and appeared, though not in the 

 viscera, but on the udder, was demonstrated conclusively 

 by the fact that before the end of five days after inoculation, 

 in the milk of the cow collected under all precautions, the 

 presence of the diphtheria bacillus could be demonstrated 

 with certainty by microscopic and culture observation ; 

 the number of bacilli present on that day in the milk 

 amounted to thirty-two per cubic centimetre. It need 

 hardly be added that these results throw a great deal of 

 light in understanding certain epidemics of milk diphtherias, 

 such as at Camberley and Yorktown, Enfield, Barking, and 

 Croydon. 



This positive result of udder eruption was also obtained 

 on two of further six experimental milch cows, and in one 

 of two cases the bacillus was demonstrated in the milk 

 about the end of the first week'"after inoculation. In all 

 cases, however, the culture used', was very virulent broth 

 culture. 



With cultures not of the virulent character — e.g. Agar 

 cultures or broth cultures of some standing, inoculation 

 produces a transitory tumour and smaller in extent without 

 the visceral disease, and the animals soon recover. Such 

 was the case in some of my own milch cows and in those 

 experimented upon by Abbott (Journal of Pathology and 

 Bacteriology, vol. ii., 1893, p. 35). 



Y 



