xill] MICROBES OF MALIGNANT ANTHRAX 325 



same size as tubercle bacilli, but a little thicker, rounded 

 at their ends, straight or sometimes more or less curved ; 

 this latter is especially noticed when they lie in groups ; 

 their substance is either homogeneous or, like that of the 

 tubercle bacilli, shows segregation of the protoplasm into 

 granules within the sheath. The bacilli stain best in 

 alkaline methylene-blue and then washed in acidulated water 

 (acetic acid i per cent.) ; also in alkaline fuchsin of Ehrlich, 

 or in gentian violet aniline water. The bacilli are easily 

 cultivated at 35-38° C. on blood serum, Agar mixture, and 



Fig. 126,— Pus of a Pulmonary Abscess in a Horse dead of Glanders. 



1. The nuclei of pus cells. 



2. The glanders-bacilli. 



Magnifying power 700. (The preparation has been stained with methylene-blue.) 



potato. On boiled potato at 35° C., they form a charac- 

 teristic yellow-brownish amber-coloured sticky film. On 

 solid blood- serum at 37° C, after three days, one notices 

 small translucent droplets slightly projecting over the 

 general surface. These are the youngest colonies. On 

 Agar culture the colonies are also translucent greyish 

 droplets, gradually flattening and becoming dark in the 

 centre. 



According to Raskina the glanders bacilli grow also at 

 18-20° C., on gelatine, milk, serum, and white of egg. 

 Kranzfeldt grew them also on glycerine Agar mixture. 



