xiii] MICROBES OF MALIGNANT ANTHRAX 



3^7 



spores. With this ngrces also the observation of Loffler, 

 that the cultures of the bacilli die after a few months, and 

 Cardeal and Malet found that putrefaction destroys the 

 bacilli, though only in many days. Loffler studied also the 

 resistance of the bacilli to heat, and he found that, for 

 instance, ten minutes' heating to 55° C. completely killed 



Fig. 127. — Film Specimen of a Pulmon'.vkv Nodule of a Hokse dead of 



Glanders ; Nur^iEROiis Glanders B.'iCILLi are shown. 



X 1000. 



the bacilli of the cultures ; in this respect the glanders 

 bacilli are even less resistant than many other non-spore- 

 bearing bacilli. Further Loffler found that perchloride of 

 mercury i : 5,000 kills the bacilli in two minutes, carbolic 

 acid (3 to 5 per cent.) in five minutes. All these facts 

 strongly point that no spore formation took place. 



Under natural conditions the general mode of infection 



