3/0 MICRO-ORGANISMS AND DISEASE [chap. 



capillar)' glass pipette a droplet of the liquefied culture 

 material is drawn up and allowed to ascend into the end of 

 the capillary glass pipette that had been pushed down into 

 the liquefied culture, or if it does not ascend is drawn up by 

 gentle aspiration at the other end of the capillary pipette ; 

 this so charged capillary tube is then withdrawn and pushed 



Fig, 147. — Stab Culture cif Bacillus of Maligkant (Edema in tre Delth of 

 Sugar Gelatine incubated fur three Da\"s at 20'" C. 



The growth is indicated by a cylinder of liquefied, slightly turbid gelatine ; on the top 



of the growth a gas bubble. 



Natural size. 



down into the lowest part of the fresh sugar gelatine tube, 

 and a trace of the material is by blowing forced out, the 

 capillary tube is withdrawn, the plug replaced, and the top 

 part of the new gelatine liquefied by holding this part of the 

 culture tube over the flame till the gelatine at this point 

 bubbles ; the tube is then placed in an upright position in 



