372 MICRO-ORGANISMS AND DISEASE [chap. 



liquefaction is proceeding along the line of growth, but at 

 greatly different rates. 



From a liquefied sugar gelatine culture a subculture is 

 easily made by rubbing a liberal amount of the material 

 over the slanting surface of solidified grape sugar Agar and 

 0-5 per cent, formate of soda (Kitasato and Weyl) ; this is 



Flu. 149- — Stab Clltl'rk of I-Jacillus Tetam in the Dei'th of Sugar 

 Gelatine, incubated for three Da^'S at 20' C. 



The growth U indicated by a spindle-shaped mass of threads extending laterally ; the 



gelatine is liquefied to the extent of the growth. 



Katutal size. 



then placed after Buchner's method in a glass tube contain- 

 ing for each gramme of pyrogallic acid one cc. of liquor 

 potassK closed by a well-fitting indiarubber plug {see a 

 former chapter) and incubated at 37' C. As soon as 

 colonies make their appearance on the Agar surface, a little 

 of it can be withdrawn by the platinum needle or loop, and 



