xv] 



ANAEROBIC BACILLI 



373 



used for flagella staining {see a former chapter), or the 

 culture can be left to go on for some time till spores have 

 made their appearance ; in some of the above microbes 

 spore-formation does not occur in the grape sugar gelatine 

 (malignant cedema, symptomatic charbon) and for this reason 

 cultures on solid media (stab culture in grape sugar Agar, or, 



Fig. 150. — Stab Culture of Bacillus of Symptomatic Charbon in Sugar 

 Gelatine, incubated for three Days at 20' C. 



The grouth is a cylinder of turbid liquefied gelatine with lateral outgrowths. 

 Natural si;^e. 



better, the just mentioned culture on the slanting surface of 

 sugar Agar) must be resorted to. 



Besides the above media, glycerine broth peptone and 

 broth peptone (ordinary nutrient broth) are useful for 

 obtaining copious growths. These are employed when it is 

 required to obtain the toxins produced by the microbe 

 during its growth. Thus in the case of bacillus of tetanus 



