XV] 



ANAEROBIC BACILLI 



375 



are sealed with gutta-percha paper immediately after 

 inoculation. 



I. Bacillus cedeinatis malig)ii (Koch). — This disease has 

 been produced by Koch in guinea-pigs by the subcutaneous 

 injection of recently manured garden earth. An extensive 

 cedema occurs at and about the seat of inoculation ; the 

 cedema is accompanied by hjemorrhage into the sub- 



FiG. 152. — Bacilli of Malignant CEdema containing Spores, chiefly 



SITUATED IN THE I\IIDDLE PaRT OF THE BaCILLI. 



cutaneous tissue, and is of an offensive odour ; it spreads 

 during the second day, leads to gangrene of the sub- 

 cutaneous and muscular tissues with the formation of gas 

 bubbles, and the animals die in from twenty-four to forty- 

 eight hours ; the spleen is found congested, so also are the 

 liver, kidney, lungs, and intestines. In the cedematous 

 exudation and in the spleen long mobile bacilli are present, 

 either singly or in filaments and long chains ; their number 



