38o MICRO-ORGANISMS AND DISEASE [chaf. 



cotton-wool, splinters soaked with the cultures — yet 

 these cultivations were always in an impure state, until 

 Kitasato {Zeitschrift f. Hygiene, Band vii., p. 225) has 

 succeeded in cultivating the tetanus bacillus of Nicolaier in 

 pure cultivations and in producing tetanus with such pure 

 cultures. Minimal doses inoculated into mice produced 

 tetanus in twenty-four hours, death in two to three da5's. 



Fig. 154. — Film Specimen of E.^cillus Tetani from a Culture tn Sugar 

 Gelatine; so.me of the Bacilli show a tekiiiinal Spore, "Drumsticks." 



In the case of rats, rabbits and guinea-pigs the dose had to be 

 somewhat larger, o-3-o'5 cc. of broth culture. Rats and 

 guinea-pigs are ill with tetanus already after twenty-four to 

 thirty hours, rabbits not before two to three days. On 

 post-mortem examination of such animals there is no sup- 

 puration at the seat of the inoculation, but only hypersemia ; 

 hence the suppuration observed in other cases is not an 

 essential feature, and in former experiments and in the case 



