386 MICRO-ORGANISMS AND DISEASE [chap. 



The infiltration with sanguineous serous fluid extends for 

 some distance into the adjacent parts in the muscular tissue ; 

 in the viscera : congestion of the liver, spleen, kidney, and 

 particularly the subcutaneous lymph glands ; these, begin- 

 ning from near the tumour, are found swollen, dark, and on 

 incision a sanguineous fluid oozes out from them. The 

 spleen is only very slightly enlarged. Cover-glass specimens 

 of the subcutaneous and muscular infiltration at or near the 

 tumour, particularly of the subcutaneous lymph glands, 

 show in considerable numbers small motile bacilli 3-5 /* 

 long, and about o's /a, thick : they are rounded at their ends, 

 and some contain terminally a bright oval spore ; others 

 possess a terminal enlargement without spore (Bollinger, 

 Arloing, Cornevin, and Thomas^). 



Immediately after death the bacilli are not easily demon- 

 strable in the heart's blood because present only in small 

 numbers, though they can be shown to be present in the 

 liver, spleen, and kidney, but always more numerously if 

 some hours are allowed to elapse after death. 



The exudation of the tumour or the surrounding muscular 

 tissue injected subcutaneously into guinea-pigs in compara- 

 tively large quantities (-|-i Pravaz syringe) produces the same 

 kind of emphysematous gangrenous change with sanguineo- 

 serous exudation at or near the place of inoculation ; the 

 animals die between twenty-four to sixty hours, the internal 

 viscera show great congestion ; in the subcutaneous tumour, 

 in the blood of the heart, and in the juice of the viscera the 

 bacilli can be easily demonstrated ; in the blood and viscera 

 they are fairly numerous if some hours have elapsed after 

 death. Rabbits are far less susceptible than guinea-pigs. 



If only a drop or two are injected the guinea-pigs, though 

 they become affected with the local disease, do not succumb, 

 ^ Bull, de fAcad, Franfaise, 1881. 



