430 MICRO-ORGANIS^IS AND DISEASE [chap. 



bouillon ; this distribution is then poured into a sterile watch- 

 glass or capsule, and -\, J, -J, j\, or y\ or less of the culture, as 

 the case requires, is drawn up into a hypodermic sterile sj-ringe 

 and injected intraperitoneally into a guinea-pig of known 

 weight. The result is always that according to the virulence 

 and the relative iiroportiou of the dose and body-weight the 



Fig. 177. — FtLM Spfxi^ien of the Peritone.al Fi.i'id of a Guinea-pig dead 

 FKor.! Acute Peritonitis afier intr.ai'eritone.vl I.\jection- i:if Cholera 

 Vibrios. 



X lOOQ. 



guinea-pig is distinctly ill after from a few to several hours, 

 the animal is quiet, does not feed, its coat becomes rough, 

 the temperature gradually falls, movement becomes more and 

 more impaired, and the animal is found dead after sixteen, 

 eighteen, twenty, twenty-four hours, or as late as thirty-six 

 hours. If it does not die after thirty-six hours it as a rule 

 again recovers. 



