XVI] VIBRIO AND SPIRILLUM 457 



in gelatine than the typical cholera vibrio ; on Agar the 

 growth is also slower and much more transparent, it does 

 not give the cholera red reaction with pure sulphuric acid 

 when grown in pure peptone salt, and when injected into the 

 peritoneal cavity of the guinea-pig is far less virulent than 

 the typical cholera vibrio. The epidemic of cholerine in 

 Lisbon had a very low mortality — few cases of death out 



Fig. 182. — FiL.M Specimen of a Flocculus from the W^ater of the polluted 

 Well of Ashbourne that hau caused an Epidemic oy Asiatic Cholera. 



of over 140 attacks, the normal mortality in an epidemic of 

 cholera being 50 per cent, and sometimes more — all these 

 facts justified Pestana in declaring that the vibrio is not the 

 cholera vibrio and the disease is not Asiatic cholera. 

 Recent observations, which I have carried out for the 

 Medical Department of the Local Government Board, and 

 which will be published in their Reports in 1896, seem to show 



