254 TEXT-BOOK OF BACTERIOLOGY. 



In staining', the glanders bacillus appears to belong to that class 

 of micro-organisms already mentioned which absorb the coloring 

 matter quickly, but readily lose it again when we decolor. Many 

 plans have been tried to overcome this difflcultj', so that for stain- 

 ing this species a long list of instructions might be formed. 



For cover-glass preparations Loffler recommends his anilin- 

 gentian-violet or anilin-fuchsin, allowing the hot liquid to act for 

 about five minutes. Then the decoloration is effected in 1^ solution 

 of acetic acid, to which the yellow color of Rhenish wine has been 

 given by adding trop^olin in aqueous solution. In this the prepara- 

 tions remain one hour and are then rinsed in distilled water. 



The same result may be more readily obtained by first treating 

 the cover-glasses with warm carbol-fuchsin or Kiihne's carbol- 

 methj'1-blue, and then only with distilled or slightlj'-acidulated 

 water — ten drops of hydrochloric acid to 500 of water. 



The double staining of the glanders bacilli has not yet proved 

 possible, and neither the process adapted to the tubercle bacilli 

 nor Gram's method is applicable. 



As the glanders bacilli only thrive at high temperatures, they 

 cannot be cultivated on gelatin. On plates of ordinary agar, or 

 agar with an addition of 4^ glj^cerin, and l?;ept at about 37° C, the 

 colonies are found abundantly developed on the second day, appear- 

 ing as light-yellow or whitish transparent, roundish accumulations. 

 The microscope shows brownish-yellow, dense, somewhat granulated 

 masses, with edges comparativelj' smooth and sharp. 



In the test-tube on oblique agar, or, better still, on glycerin agar, 

 a clearly-defined whitish, translucent, moist shining coat forms in 

 four or five days along the inoculation scratch at incubator heat. 

 On blood-serum, generally in the same length of time, spots appear 

 here and there, which are roundish, perfectly transparent, more or 

 less yellow, and drop-like, and do not liquefy the medium. After- 

 ward they coalesce into one even, tough, slimy covering. 



The growth of the glanders bacilli on potatoes is very charac- 

 teristic and in many respects worthj' of note. Shortly after the 

 inoculation on slices prepared after Globig's method and kept at 

 incubator heat, an amber-yellow, curiously-transparent covering 

 appears, which looks almost like a thin laj^er of honey, but which 

 increases rapidly in thickness and at the same time assumes a 

 darker tint. At the end of a week the culture is reddish-brown or 

 fox-red, and presents such a peculiar appearance as almost to pre- 

 vent the possibility of its being confounded with any other species. 



From the agar, blood-serum, and also from the potato success- 

 ful transmissions can be made without difficulty, and a small trace 



