CONTENTS. 



PAGE 



Pbepacb 5 



1. Bacteriological TECHNiquE 7 



2. Staining Methods 13 



I. Staining Bacteria in Cover-glass Preparations with Watery 



Solutions of Aniline Dyes 13 



II. Staining Bacteria in Sections with Watery Solutions of Ani- 

 line Dyes 17 



III. Gram-Gnnther Method of Staining Bacteria in Sections . . 19 



IV. Gram Method of Staining Bacteria in Cover-glass Preparations 21 

 V. Ziehl-Gabbet Method of Staining Tubercle Bacilli in Cover- 

 glass Preparations 23 



VI. Koch-Ehrlich Method of Staining Tubercle Bacilli in Cover- 

 glass Preparations 25 



VII. Eoch-Ehrlich Method of Staining Tubercle Bacilli in Sections 27 



VIII. Arens' Method of Staining Tubercle Bacilli 29 



IX. Staining Tubercle Bacilli in Sections with Ziehl's Carbol- 



Fuch.'iin Solution 31 



X. Weigert's Fibrin or Bacteria Stain in Sections 33 



XI. Staining the Glanders Bacillus 35 



XII. Staining Spores, especially Anthrax Spores 39 



XIII. Moller's Method of Staining Spores 41 



XIV. Staining Sections with Bismarck-Brown, Loeffler's Alkaline 



Methylene-blue, or Watery Solutions of the Aniline Dyes . 43 

 XV. Double Staining of Sections with Hematoxylin and Picric 



Acid or Eosin 45 



XVI. Typical Method of Staining Anthrax Bacilli from the Blood 



in Cover-glass Preparations 49 



3. Preparations of Nutrient Media 53 



4. Imbedding Tissues for Cutting Sections 59 



3 



