n. STAINING BACTERIA JN SECTIONS WITH WA- 

 TERY SOLUTIONS OF THE ANILINE DYES.' 



1. Section from alcohol into water, one minute. 



2. In staining fluid,^ two minutes to five minutes. 



3. Decolorize in acidulated water (acetic acid 1 :1000), 

 one minute. 



4. Wash in eighty per cent, alcohol; then wash and 

 dehydrate in absolute alcohol until no more coloring mat- 

 ter comes away. 



5. Clear in oil of cloves, cedar-oU, or oil of origanum. 



6. Transfer to the slide, with a spatula or with cigar- 

 ette paper, and absorb superfluous oil with filter-paper. 



7. Bring a drop of xylol-balsam upon the section and 

 place a cover-glass upon it. 



iFor the bacteria mentioned under I. 

 2See I, foot-note 2, a and b. 



17 



