Vm. ARBNS' METHOD OP STAINING TUBERCLE 

 BACILLI/ 



(A). IN OOVEE-GLASS PREPARATIONS. 



1. Stain from four to six minutes in chloroform- 

 f uchsin solution.^ 



2. Decolorize in 96 per cent, alcohol + -3 drops HCl to a 

 watch-glass. 



3. Wash in water. 



4. Contrast stain with methylene-blue, etc. 



(B) IN SECTIONS. 



1. Sections from alcohol to chloroform-fuchsln solu- 

 tion, four to six minutes. 



2. Decolorize as under A 2. 



3. Wash in water. 



4. Contrast stain, etc. 



JRecommended for staining these bacilli in fatty substances, 

 milk, etc. 



2CHLOROFORM-FUCHSIN SOLUTION: 



(a) one or two crystals of fuchsin dissolved in from 2 to 3 co 

 chloroform, or (b) concentrated alcohol solution fuchsin, 3 to 4 drops. 



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