IX. STAINING TUBERCLE BACILLI IN SECTIONS 

 WITH ZIBHL'S CAEBOL-FUCHSIN SOLUTION. 



(A) ALCOHOL SECTIONS. 



1. Stain from ten to twenty minutes in Ziehl'a car- 

 bol-fuchsin solution (See V, foot-note 1). 



2. Same as VH, 2 to 6. 



(B) FROZEN SECTIONS. 



(a) 1. Sections from salt-solution to 80 or 96 per cent, 

 alcohol for about fire minutes to harden. That the sec- 

 tion may remain smooth, it is best to transfer it as fol- 

 lows: Lift from the salt-solution with a spatula; drain off 

 superfluous fluid; add 1 to 2 drops of alcohol with the 

 end of a glass rod, and after a moment slip off into alcohol. 



2. Same as above under A, or 



(b) 1. Sections from salt-solution to water. 



2. Harden in corrosive sublimate (1:1000 to 1:500) 

 from one-half to one hour. 



3. Wash in water and proceed as above under A. 



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