Xm, HOLLER'S METHOD OP STAINING SPORES. 



1. Dry and fix the coyer-glass preparation. 



2. In chloroform, two minutes. 



3. Wash in water. 



4. In 5 per cent, chromic acid, for a half to two min- 

 utes. 



5. Water. 



6. Flood the glass with Ziehl's carbol-fuchsin solu- 

 tion ; boil and keep very hot for one minute. 



7. Wash in water. 



8. Decolorize in 5 per cent. HjSO^ for from five to 

 ten seconds. 



9. Wash in water. 



10. Stain with methylene-blue. 



11. Wash in water, mount, etc. 



41 



